Selection and Verification of Reference Genes for qRT-PCR Analysis in Iris domestica under Drought

Iris domestica is a plant of the Iridaceae family and drought-tolerant, but its drought-resistance mechanism not yet clear. Analysing gene expression changes I. by qRT-PCR an important mean to understand drought resistance characteristics. Nevertheless, lack reference genes greatly hinders investigation research on adaptation at molecular genetic levels. In this study, we assessed stability 11 candidate in under stress conditions different tissues using geNorm, NormFinder, BestKeeper RefFinder tools. The results showed that EF1β was most stable tissues. To validate further identified genes, patterns VP analysed. These will be conducive more accurate quantification levels domestica.